High-throughput mapping of a whole rhesus monkey brain at micrometer resolution

Fang Xu, Yan Shen, Lufeng Ding, Chao-Yu Yang, Heng Tan, Hao Wang, Qingyuan Zhu, Rui Xu, Fengyi Wu, Yanyang Xiao, Cheng Xu, Qianwei Li, Peng Su, Li I Zhang, Hong-Wei Dong, Robert Desimone, Fuqiang Xu, Xintian Hu, Pak-Ming Lau, Guo-Qiang Bi. Nature biotechnology, Jul 2021.


Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and the relatively low throughput of available microscopy methods. Here, we present an approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed fluorescence microscopy implementing improved volumetric imaging with synchronized on-the-fly-scan and readout technique, and is capable of completing whole-brain imaging of a rhesus monkey at 1 × 1 × 2.5 µm³ voxel resolution within 100 h….