Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

Leah C Acker, Erica N Pino, Edward S Boyden, Robert Desimone. Journal of visualized experiments : JoVE, Oct 2017.


This protocol describes a large-volume illuminator, which was developed for optogenetic manipulations in the non-human primate brain. The illuminator is a modified plastic optical fiber with etched tip, such that the light emitting surface area is > 100x that of a conventional fiber. In addition to describing the construction of the large-volume illuminator, this protocol details the quality-control calibration used to ensure even light distribution. Further, this protocol describes techniques…